The most common oligosaccharides in human milk are 3-fucosyllactose (3FL) (0.07-0.86 g/L), lacto-N-tetraose (LNT) (0.18-1.18 g/L), lacto-N-neotetraose (LNnT) (0.10-2.04 g/L), 2′-fucosyllactose (2′FL) (0.45-4.04 g/L), lacto-N-fucopentaose I-III (LNFP I-III), and lacto-N-difucohexaose I-II (LNDFH I-II). They play an important role in infants' growth, which includes enhancing the immunity and regulating microflora in the gut. At present, the main analytical methods for oligosaccharides in human include high performance liquid chromatography (HPLC) and high performance anion exchange chromatography with pulsed electrochemical detection (HPAEC-PED). This paper reviews the content and analytical method of common oligosaccharides in human milk.