To investigate the glycerol content of 93 domestic wine samples from eight major growing region of China and 4 suspicious poor quality wines and offer important reference for the quality identification.Methods The glycerol content in wines was determined using glycerol enzymatic kits and alcoholic strength was tested according to GB 15038-2006. The results of glycerol content range and the ratio of glycerol/alcohol were analyzed. Results The glycerol content of wine was affected by many factor including climate, grape varieties and grape maturity. The glycerol content ranged from 4-15 g/L and the ratio of glycerol/alcohol ranged from 6%-10% in normal fermentation. Most of the wines were in the normal range. If the results were abnormal, other analysis methods should be taken for further testing. Conclusion The glycerol content combined with the ratio of glycerol/alcohol gave some useful clue of wine quality, and inspection and supervision should be strengthened.