Abstract:To evaluate and validate the performance of the RapidChek SELECT method for rapid detection of Salmonella in foods.Methods The limit of detection (LOD) and the specificity of RapidChek SELECT method were assessed by spiking Salmonella and non-Salmonella reference cultures into different food matrix, respectively. Meanwhile, RapidChek SELECT methodwas validated via examining Salmonella contamination in food samples in comparison with the China national standardmethod. Results The limit of detection of RapidChek SELECT method were 1 cfu/25 g or 1 cfu/ml. The RapidChek SELECT method showed good specificity and no cross-reactivity in determination of 10 kinds of non-Salmonella reference strains. The lowest cell density of Salmonella in food samples for direct detection by RapidChek strip was 106 cfu/ml. Of the 40 food samples examined, 87.5% (35/40) and 85% (34/40) were positive for Salmonella examined by RapidChek SELECT method and China national standardmethod, respectively. A good coincidence in the results obtained by both methods was observed (97.5%, 39/40). RapidChek SELECT method exhibits a slightly better proficiency than China national standardmethod.Conclusion The RapidChek SELECT is an effective alternative analytical method with characteristics of high sensitivity, specificity, easy-to-use, lower interference from non-Salmonella, time-saving, and suitable for rapid detection of Salmonella in foods.