To understand the status of lead, cadmium and methylmercury residue in seafood collected from Zhoushan City, meanwhile, make comparisons and evaluation.Methods The lead, cadmium and methylmercury residues in sea fish, mollusks and shellfish from Zhoushan City were detected using single variance analysis. Results The average content of lead, cadmium and methylmereury in sea fish were 0.166 7,0.008 3 and 0.031 3 mg/kg , 0.145 1,0.055 9 and 0.009 1 mg/kg in mollusks, and 0.063 6,0.053 7 and 0.015 4 mg/kg in shellfish. The content of lead in sea fish was higher than that of the mollusks and shellfish, the content of cadmium in mollusks was higher than that of the shellfish and sea fish, the content of methylmercury in sea fish was higher than that of the shellfish and mollusks, and the differences were all significant (P<0.05).Conclusion Overall seafood of Zhoushan were qualified, only a few sea fish and shellfish samples exceeded the lead and cadmium limits.