To investigate the stability of natural vitamin E coenzyme Q₁₀ soft capsules with different formula.Methods The content of coenzyme Q10 and vitamin E of coenzyme Q₁₀ soft capsule samples with different formulas were analyzed quantitatively with high performance liquid chromatogram (HPLC) and gas chromatogram (GC) respectively. At the same time, the existing forms of coenzyme Q₁₀ were also determined. Results In the formulas with only coenzyme Q₁₀ or coenzyme Q₁₀ combined with vitamin E (d-α-tocopherol acetate) as active ingredients, the existing form of coenzyme Q₁₀ was oxidized form (CoQ₁₀), both coenzyme Q₁₀ and d-α-tocopherol acetate were stable during the accelerated stability test. In the formula with coenzyme Q₁₀ combined with vitamin E (d-α-tocopherol) as active ingredients, both oxidized coenzyme Q₁₀ (CoQ₁₀) and reduced coenzyme Q₁₀ (CoQ₁₀H₂) existed, CoQ10 content decreased and CoQ₁₀H₂ content increased, the total amount showed no significant difference before and after the accelerated stability test, while the d-α-tocopherol amount reduced to a certain degree.Conclusion The formulas with only coenzyme Q₁₀ or coenzyme Q₁₀ combined with vitamin E (d-α-tocopherol acetate) were stable. In the formula with coenzyme Q₁₀ combined with vitamin E (d-α-tocopherol), CoQ₁₀ would be converted into CoQ₁₀H₂ and the d-α-tocopherol content decrease.