To analyze the similarity of molecular characters and drug resistance of non-typhoidal Salmonella isolates from commercial chickens and patients with diarrhea, and to prevent the infection of Salmonella.Methods93 Salmonella strains were isolated and serotyped from 1 054 stool specimens of diarrhea patients and 440 anal swab of live chickens from markets which were collected from January 2012 to October 2013. Common non-typhoidal Salmonella were molecular classified by pulsed field gel electrophoresis (PFGE), clustering analysis was conducted by Bionumerisc v4.0 and drug sensitivity test was conducted by K-B method. Results88 strains of non-typhoidal Salmonella were detected in 1 054 diarrhea specimens, with the detection rate of 8.35%. The strains were subtyped into 25 serotype, and Salmonella enteritis was the majority. 46 strains of hon- typhoidal Salmonella were detected from patients under the age of 2 accounting for 52.27%. The PFGE showed that 16 strains of Salmonella enteritidis could be divided into 14 patterns, 12 strains of Salmonella stanley could be divided into 11 patterns, 14 strains of Salmonella typhimurium could be divided into 14 patterns and 6 strains of Salmonella derby could be divided into 5 patterns. The similarity between the strains were compared and two PFGE patterns of Salmonella enteritidis were 100% homologous, while there was little similarity in other strains. Meanwhile, there was little similarity between strains from chicken and patients. The drug resistance rate of non-typhoidal Salmonella derby to 12 kinds of antibiotics was below 50%. The drug resistance rate of different serotype was not identical, and Salmonella derby had a high resistance. 5 strains of non-typhoidal Salmonella were isolated from 440 anal swab in chicken with the detection rate of 1.14%. The strains were divided into 4 serotype, and Salmonella mbandaka was not detected in patients.ConclusionThe rate of diarrhea caused by non-typhoidal Salmonella was high in this region, especially in infants and young children. Strains from patients and chickens did not have the same clone so that common antibiotics had a good sensitivity.