To explore the wild mushroom poisoning space-time distribution characteristics in Yunnan Province in 2005-2013 with geographic information system, and provide scientific evidence for prevention strategy.MethodsDescriptive epidemiology was used to analyze the wild mushroom poisoning events in Yunnan province. MapInfo 7.8 software was used to make thematic map. A global means of spatial autocorrelation with Moran's I was used to explore the whole spatial distribution pattern of the wild mushroom poisoning incidence in Yunnan Province. The location and intensity of cluster were detected by the local spatial autocorrelation statistic.ResultsThere were 211 wild mushroom poisoning events reported from 2005 to 2013. The poisoning peak occurred in 5-9th month annually. Local spatial autocorrelation analysis revealed several counties had cluster such as Guangnan county, Wenshan county, Yanshan county, Qiubei county, Lancang county, Menglian county, Menghai county, and Jinghong county. Poisoning area was still gradually expanding to the northwest and northeast.ConclusionThe wild mushroom poisoning events prevalence was obviously distributed seasonally and spatially in Yunnan Province and effective measures should be taken to control its prevalence.