Typing Listeria monocytogenes isolated from food by DiversiLab system
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(Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100026,China)
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Abstract:
L.monocytogenes isolates were typed by DiversiLab system. The result was compared with the results of serotype and the antibiotic resistance.MethodsThe DiversiLab system was based on the principle of rep-PCR. ResultsThe 46 isolates were classified as nine types, type A to I. The 1/2a serotype isolates mainly belonged to A type. Six 4b serotype isolates belonged to H type. Among 14 nitrofurantoin resistance isolates, nine of them were A type, one isolate was B type, three isolates were C type, and one isolate was F type. Most of the nitrofurantoin resistance isolates were 1/2a serotype, except two of them were 3a serotype.ConclusionDiversiLab system typing results revealed the genetic characters and relationship of 46 foodborne L.monocytogenes. The typing results related to the H sites of serotype. The similarity of 14 nitrofurantoin resistance isolates was high, and related to anti-nitrofurantoin resistance genes.
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ZENG Jing, ZHOU Xue-ting, ZHANG Xi-quan, ZHANG Lin, ZHANG Xi-meng, WEI Hai-yan, ZHOU Xi-cheng, MA Dan, NI Yuan-ying. Typing Listeria monocytogenes isolated from food by DiversiLab system[J].中国食品卫生杂志,2014,26(6):532-536.