To detect the pathogens of food poisoning samples and analyze the homology, help to trace the sources of contamination and clarify etiology diagnosis, and provide the basis for the prevention and control of food poisoning.MethodsThe pathogens were screened by fluorescence quantitative PCR , isolated according to the GB method, identified by ATB method, and the homology was analyzed by PFGE.Results8 strains of Salmonella enteritis were separated from 21 patients or operators, 2 strains were from 9 food samples. The detection rate were 25.00% and 6.25% respectively. Salmonella was not detected from water samples from canteen and well. Positive rates were the same for real-time fluorescent quantitative PCR and GB method. PFGE patterns of the 10 Salmonella enteritis were the same for cluster analysis.ConclusionThe food poisoning case was caused by the same clone of Salmonella enteritis. Real-time fluorescent quantitative PCR was helpful for rapid examination for pathogenic bacteria. GB method was important for the separation of Salmonella enteritis, and the above 2 methods could increase the detection rate and shorten the time spent. PFGE method could analyze the homology of the pathogenic bacteria, trace the source and was helpful to prevent and control food poisoning.