To develop a genotyping and tracing method for foodborne pathogenic Staphylococcus aureus (S.aureus).MethodsFoodborne S. aureus isolates were identified through PCR method targeting coagulase gene and subtyped by RiboPrinter. Results62 isolates were identified as S.aureus and classified into six subtypes, and each subtype was hypodispersion.ConclusionCooperative use of PCR method and RiboPrinter method could precisely identify and classify S.aureus contamination, analyze the distribution and characteristic, and trace the possible fingerprint. This research may provide experimental data for pathogenic bacteria typing, and give technical support for food supervision and administration.