A high performance liquid chromatographic method was developed for determination of 8 fluoroquinolones residues in animal derived food (including marbofloxacin, ciprofloxacin, danofloxacin, norfloxacin, enrofloxacin, difloxacin, oxolinic acid, and flumequine).MethodsThe sample residues were extracted by EDTA-Mcllvaine buffer, purifid with PEP-2 solid-phase extrction column, seperated by ZORBAX C₁₈ (5 μm, 4.6 mm×250 mm) and quanlified by external standard calibration curves. ResultsMarbofloxacin, oxolinic acid, and flumequine in 30-360 μg/L, and the remaining five drugs in 15-180 μg/L linear relationship is good, the correlation coefficient is greater than 0.99. The detection limit (including norfloxacin, ciprofloxacin, enrofloxacin, difloxacin) is 2 μg/kg, the detection limit of danofloxacin is 0.5 μg/kg, the detection limit of marbofloxacin, oxolinic acid and flumequine is 8 μg/kg, the recoveries are between 77.6%-98.3%, the relative standard deviations are between 2.77%-9.35%.ConclusionThe method was simple, high accuracy and precision, less harmful to human body and could be satisfied with diffirent substrate test.