To determine 20 kinds of industrial dyes simultaneously in condiments with ultra high performance liquid chromatography(UPLC)．MethodsSamples were separated on a Endeavorsil C₁₈(100 mm × 2.1 mm, 1.8 μm)chromatographic column and gradient eluted of 20 mmol/L ammonium acetate aqueous solution(containing 0.1% formic acid) methyl alcohol. The wavelength of the detector was at 450,0 and 600 nm． ResultsThe calibration curve showed good linearity in the range of 2-40 μg/ml, and the correlation coefficients were better than 0.999. The limits of detection were in the range of 0.07-0.75 mg/kg. The recoveries of standard addition at three levels was 72.3%-93.0% and the RSD (n=6) was 1.0%-6.2%．ConclusionThe method was sensitive and accurate, it could be used for the detection of the 20 kinds of industrial dyes in condiments simultaneously．