A method was developed for the determination of 10 preservatives and bactericides in drinks by HPLC-DAD.MethodsThe samples were extracted by methanol and purified by solid phase extraction with a C8 SPE column. The analytes were separated on a C18 column, eluted by a gradient elution with 0.02 mol/L ammonium acetate buffer and methanol, detected by diode assay detector, and quantified by external standard method.ResultsThe calibration curve was linear within the concentration range of 0.1-10.0 μg/mL(r≥0.995 1). The lower limit of detection in drink was 0.25-1.0 μg/ml(S/N=10). The relative recoveries of 10 preservatives-bactericides in fruit juice drinks and carbonated drinks with 3 spiked levels were 74.1%-94.1%, and relative standard deviations(RSD)were 5.3%-15.1%.ConclusionThe method was simple, fast, low detection limit, meet for drinks foods at home and abroad in the above 10 kinds of antiseptic fungicide limited requirements, applicable to 10 kinds of preservatives and bactericides in drinks.