To investigate the contamination and the virulence genes of Staphylococcus aureus in the primary farm products of Minhang District, Shanghai.MethodsCHROMagar color medium and API S.aureus were used for separation and biochemical identification of S.aureus according to national standard. Virulence genes of coa,nuc,clfA,sea,seb, sec, sed and see were detected by real-time PCR methods．Results43 isolates were identified from 206 S. aureus samples,and the detection rate was 20.87%. All the identified strains had coa, nuc and clfA virulence genes, but no see virulence gene was found, and the remaining four virulence genes expressions were partially missing.ConclusionThe contamination of S.aureus in the primary farm products existed in Minhang district of Shanghai. Supervision and management should be strengthened for raw meat and poultry for serious contamination. All S.aureus strains contained coa virulence gene. It could be used as a target for detecting virulence genes.