In order to study the toxicity of the culture supernatant of Clostridium sporogenes.MethodsStrains of C.sporogenes was isolated from the whey protein concentrate (WPC) and its products. The culture supernatant of C.sporogenes reached maximum in the early stationary phase of the bacterial growth in cooked meat medium or trypticase-peptone-glucose-yeast extract broth that contained rich glucose, ammonia and peptide. The culture supernatant of C.sporogenes and treated culture supernatant are injected to ICR mice intraperitoneally or by oral perfusion. The death of ICR was recorded. ResultsIntraperitoneal (i.p.) injection of the culture supernatant caused death of ICR mice. ICR mice showed slow the poisoning symptoms, including irritable, hair arched and shortness of breath and sudden death within 10 min, the average 5 to 7 min. The toxic effect of the culture supernatant produced by C.sporogenes is different from botulinum toxin poisoning.ConclusionWith the notable exception of production of botulinum neurotoxin, the isolation of C.sporogenes from WPC and its products suggest that the culture supernatant of C.sporogenes have the potential toxicity to be present in mice.