To investigate the effect of the flavonoids-rich fruit and vegetable juice intake on the oxidative damage related phase Ⅱ enzyme gene expression in rat liver.Methods54 healthy Wistar rats (SPF, male) were randomly divided into control group, lower dosage fruit and vegetable juice group and higher dosage fruit and vegetable juice group. The control group was treated with saline water and the latter two groups were treated with different dosage of fruit and vegetable juice for 5 weeks. Rat serums were obtained for the measurement of oxidative damage related biomarkers. Liver tissues were used for the detection of phase Ⅱ enzyme gene expression. The oxidative damage related phase Ⅱ enzyme gene mRNA expression was detected by RT-PCR and the serum T-AOC, MDA and GSH levels were tested by using experimental kits. ResultsComparing with the control group, fruit and vegetable juice increased the serum antioxidant capacity significantly, as well as up-regulated the mRNA expression of NQO1 and GCLC genes (P<0.05) in rat liver tissues. However, the dietary intervention had no effects on the mRNA expression of GSTP1, GCLM, GSTM2 and GSTA2 (P<0.05).ConclusionFlavonoids-rich fruit and vegetable dietary intervention improve the antioxidant capacity and the up-regulation of NQO1 and GCLC gene mRNA expression in rats.