Survey and hemolysin gene analysis of Bacillus cereus in infant food fom Shijiazhuang market
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(Shijiazhuang Center for Diserse Control and Prevention,Hebei Shijiazhuang 050011,China)

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    Abstract:

    To investigate the contamination of Bacillus cereus in infant formula milk powder and cereal-based complementary foods from Shijiazhuang market, and also to monitor the toxin genes of the isolated strains.MethodsInfant formula powder and cereal-based complementary foods from 23 counties of Shijiazhuang city were collected. Bacillus cereus strains were tested and counted according to the national standard GB/T 4789.14-2003 and foodborne pathogenic bacteria monitoring manual. The hemolytic toxin genes and non-hemolytic toxin genes of isolates were detected by real-time PCR. Results85 strains of Bacillus cereus were detected from 399 samples. The detection rate of infant formula powde was 20.3%(36/158) and cereal-based complemenary foods was 22.8%(49/241). The hemolysin gene was positive in 48 isolated strains and the detection rate was 56.5% with no isolates carrying non-hemolytic toxin gene.ConclusionThe contamination of Bacillus cereus in infant food was quite severe. These foods had a potential food poisoning risk. Our results could provide reference for the infant food hygiene inspection standard setting, supervision and management.

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QIN Li-yun, LV Guo-ping, GUO Yu-mei, QI Hui-rong, PAN Zhuo. Survey and hemolysin gene analysis of Bacillus cereus in infant food fom Shijiazhuang market[J].中国食品卫生杂志,2014,26(4):388-390.

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  • Received:February 14,2014
  • Revised:
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  • Online: August 15,2014
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