Purification of anti-Ara h1 antibody from peanut allergy serum
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(Allergic Reactions and Immunology Research Institute of Shenzhen University,Guangdong Shenzhen 518060,China)
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Abstract:
Ara h1 is a major allergen proteins in peanut, which can make peanut allergic patients produce specific IgG and IgE. Ara h1 and the specific binding of IgG and IgE is an important cause of peanut allergy. ProteinA affinity chromatography and affinity chromatography with Ara h1 as a ligand were used to purify anti-Ara h1 IgG and IgE. Purified specific IgG and IgE antibodies have important significance for the study of allergen and antybody interactions.
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HE Wei-yi, LI Yao, JING Jiang-shan, FENG Yue, XING Peng, HUANG Hai-zhen, WU Xu-li, LIU Zhi-gang. Purification of anti-Ara h1 antibody from peanut allergy serum[J].中国食品卫生杂志,2014,26(4):340-343.