Survey of drug resistance and virulence gene of Vibrio parahaemolyticus from imported aquatic products
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(Ningbo College of Health Sciences,Zhejiang Ningbo 315100,China)

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    Abstract:

    To survey the drug resistance and the distribution of virulence gene in isolated Vibrio parahaemolyticus (VP) from the imported aquatic products at Ningbo port.MethodsThe 129 VP strains were isolated from imported aquatic products at Ningbo port and 15 antibiotics were used for drug resistance test by K-B test method. Polymerase chain reaction was used for testing the 4 virulence genes(tlh、trh、tdh、toxR)from VP.ResultsThe drug resistance rates of ampicillin, carbenicillin and cefradine were 90.70%, 98.45% and 51.17%, respectively. The drug resistance rates of kanamycin, cefalexin, cephazoline and cefuroxime were 1.55%, 13.18%, 2.33% and 20.16%, respectively. Medium sensitive rates of kanamycin, cefalexin, cephazoline and cefuroxime were 33.33%, 52.71%, 46.51%, 40.31%, respectively. All strains were sensitive to the 8 antibiotics (Tetracycline, doxycycline, amikacin, gentamicin, cefoperazone, ofloxacin, chloramphenicol, co-trimoxazole). The detection rates of trh, tdh, tlh and toxR genes were 0%, 0.78%, 100% and 100%, respectively.ConclusionThe doxycycline, gentamicin, co-trimoxazole were the preferred antibiotic for people infected by VP from the imported aquatic products. The rate of VP strains carrying main virulence gene was relatively low in VP strains in the imported aquatic products.

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YU Ji-mian, NI Jing-jing, TAO Dong-ying, LAO Hua-jun, WANG Jian-feng, WANG Chun. Survey of drug resistance and virulence gene of Vibrio parahaemolyticus from imported aquatic products[J].中国食品卫生杂志,2014,26(4):320-323.

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History
  • Received:May 27,2014
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  • Online: August 15,2014
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