To improve the ability of rapid detection and tracing the infectious source of food poisoning outbreak with molecular biological technology.MethodsRapid detection of 10 fecal samples and 6 food samples were carried out with real-time fluorescent PCR. A total of 39 fecal samples and 8 food samples were detected by culture, isolation and identification method. Virulence gene of invA was detected by PCR. Homologous relationship among strains from local region and other regions was analyzed with PFGE and MLST genotyping technique respectively. ResultsSalmonella was detected in 10 fecal samples and 6 food samples with real-time fluorescent PCR. Thirty-one strains of Salmonella enteritidis were finally isolated and identified in 39 fecal samples and 8 food samples. Homology among those 31 strains was proved by PFGE, which suggested that the strains from food samples and clinical samples were from the same origin. Homology among the isolates and prevalent strains in other regions was also shown with MLST genotyping results. Virulence gene of invA in all isolates was testified by PCR. ConclusionTimeliness and accuracy of strain identification during food poisoning incident could been improved by real-time fluorescent PCR. Combination of PFGE and MLST genotyping techniques was of great significance to trace homologous relationship among strains in local region and other regions, and to understand their epidemiological characteristics.