An ultra high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-MS/MS) method was developed for the determination of α-solanine and α-chaconine in potato tuber.MethodsThe sample was extracted with 5% acetic aicd and purified with C₁₈ SPE column(600 cc). The extraction was separated on a waters BEH HILIC column (100 mm ×2.1 mm,1.7 μm) using 88% acetonitrile with 10 mmol/L ammonium acetate as mobile phase at 0.3 ml/min. The detection was performed with waters Xevo TQ-S using postive electrospray ionization (ESI⁺) mode and multiple-reaction monitoring (MRM) mode.ResultsThe results showed that the linear range for α-solanine was 0.5-500 ng/ml. The LOQ for α-solanine was 0.7 and 0.35 μg/kg for α-chaconine. The method showed good accuracy with RSD<10% (n=6) and recovery>80%.ConclusionThis method is fast, simple and has good reproducibility. It is suitable for α-solanine and α-chaconine determination in potato tuber and crisps.