To investigate the skin antiaging efficacy of plant antioxidant blend based on the concept of non-animal testing.MethodsHaCaT keratinocyte cell line were induced oxidation damage by ultraviolet irradiation and treated with resveratrol, procyanidins and two substances blend respectively. The quantitative measurement including cell viability, ROS, SOD, cell cycle and cell apoptosis were detected by MTT assay, fluorescein labeling and ELISA method. The ocular irritation of eye cream formula containing plant material were assessed by combination of CAMVA and BCOP testing. Clinical efficacy was evaluated in terms of chronoaging and photoaging features including skin hydration, elasticity and color by non-invasive methods on 25 female volunteers after 60 days of treatment. ResultsResveratrol-procyanidins blend antioxidant could significantly relieve HaCaT cell oxidative lesion induced by UV. The SOD level in test group were significantly higher than radiation group (P<0.001). All ROS fluorescence intensity, apoptosis cell and the percentage of S-phase cell in cell cycle were obviously decreased in test group (P<0.001). The antioxidant ability of blend are better than resveratrol and procyanidins. The clinical test of volunteers after alternative combination evaluation of its safety, which showed that skin moisturization, TEWL, elasticity and color had significantly improved. ConclusionSpecific plant antioxidant blend by resveratrol and procyanidins could effectively reduce skin oxidative stress and improve function.