To analyze the ability and accuracy of protein microarray for body iron stores (BIS) and iron deficiency diagnosis.MethodsThe protein microarray was compared with commercially available traditional tests for serum ferritin and soluble transferrin receptor, evaluated by indicator including the sensitivity, specificity, Youden's index, likelihood ratio, Kappa index. The BIS results were compared between protein microarray and immunoturbidimetry by paired t test. ResultsThe protein microarry showed higher sensitivity, better specifcity and coherence with immunoturbidimetry on iron deficiency estimates. At the same time, more BIS insufficient were discoverd by protein microarray.ConclusionThe protein microarray, a new rapid diagnosis technique, could be used to simultaneously detect SF and sTfR, opposed high diagnosis potencial on iron deficiencyestimates and it was valuable for more application research.