To establish a quick checking method of enterotoxins genes in food-borne Listeria monocytogenes by multiplex polymerase chain reaction (MPCR) and denaturing high-performance liquid chromatography (DHPLC).MethodsPrimers for detection of hly, prfA, inlA and inlB were designed and PCR system was optimized. Products were detected by DHPLC for quick detection. Thirty-two strains were tested with PCR method, sensitivity was determined with various concentration of standard strains. ResultsThe peak order of PCR products was inlB, hly, inlA, prfA, and amplified fragment size was 146,0, 255and 388bp respectively. This method has good specificity, and the lowest amount of detecting was 280cfu/ml.ConclusionThis method can well meet the requirements of actual food microbe testing.