To developea method for the separation and determination of chromium by the cloud point extraction-graphite furnace atomic absorption spectrometry(CPE-GFAAS).MethodsUsing Triton X-100as surfactant and 8-hydroxyquinoline(8-HQ) as complexing agent, the cloud point extraction rate of the trivalent chromium with graphite furnace atomic absorption spectrometry(GFAAS)was optimized bypH,Triton X-100dosage, temperature, time and other majorfactors. The content of Cr(Ⅵ) was determined by total chromium and Cr(Ⅲ). ResultsThe optimum cloud point extraction condition was pH=9,1.0ml 3% Triton X-100, equilibrium temperature 95℃, equilibrium time 20min. Under this optimum condition, the limit of detection was 0.2μg/L and relative RSD was 4.1%. The spiked recoveries were above 88%-98%.ConclusionThis method has the advantages of low pollution,convenience,accuracy,high sensitivity and high precision．