To confirm the feasibility of BN rats as the protein allergy animal model．Methods48female BN rats were randomly divided into 6groups, control group, PAP group, HEWP group, OVA-low group, OVA-middle group, OVA-high group, 8rats in each group. The above groups were administrated 1ml sterilized water, 1mg/ml PAP, 10mg/ml HEWP, 0.1mg/ml OVA, 1mg/ml OVA or 10mg/ml OVA solution, respectively daily for 6weeks by gavage. Blood samples were taken on the 14^th,28^th and 42^nd days from the orbital plexus,and centrifuged to obtain serum for analyzing specific IgG and total IgE．On the 21^st and 35^th days,blood samples were taken to obtain plasma for analyzing histamine．In addition,the changes of blood pressure and gut permeability were determined．ResultsDifferent concentrations of allergen OVA could stimulate allergic reaction in BN rats, including elevated specific IgG and total serum IgE, elevated histamine level, decreased blood pressure, where 1mg/ml OVA was the optimal sensitizing dose. The allergic reactions to Weak allergens (HEWP) was weak, allergic reactions to non allergen (PAP) was negative. Gastrointestinal function had no obvious physiological changesfor all groups.ConclusionBN rat could be a suitable animal model for evaluation of food protein allergy．