To investigate the situation of Salmonella contamination and serotype distribution isolated from poultry and livestock in Kaifeng city, and to provide a scientific basis for foodborne diseases prevention and control.MethodsSamples of poultry and livestock were collected periodically and randomly from the slaughterhouses and farms in Kaifeng city. Samples were detected according to GB 4789.4-2010and the handbook of investigation of food contamination bacteria of Chinese CDC in 2011. ResultsAmong 691samples, 248were detected positive for Salmonella with a detection rate of 35.9%. 253strains of Salmonella were isolated, belonging to 17serotypes.ConclusionIn Kaifeng city, the poultry and pork, particularly fresh chicken, were contaminated by Salmonella at certain degree. Enhanced management and monitoring in breeding, slaughtering, processing of poultry and livestock are needed to reduce the incidence of food-borne infections.