The aim of the study was to investigate the hygiene and sanitation of seafood enterprises according to Enterococcus in processing water.MethodsAccording to the method of ISO7899-2∶2000(Water quality-Detection and enumeration of Enterococcus-Part 2:Membrane filtration method), Enterococcus was isolated and identified. Primers and probes were designed and synthesized targeting tuf gene.Results The results showed that 14.6% of the 41isolates were Enterococcus positive. Most of them were GroupⅡ strains. Conclusion We should take effective measures of prevention and control because the health status was not satisfying.