To study the bacteria contamination in frozen intestines.Methods Sixty-five strains of bacteria isolated from frozen intestines, which collected in Nantong casings manufacturing and processing enterprises between 2010and 2012, were identified by 16S rDNA PCR amplification and sequence analysis.Results It showed that 20specices of bacteria blonging to 13genuses were identified,and the main bacterial species were Proteus mirabils, Enterococcus hirae and Enterococcus faecalis.In addition, some of them may cause disease in humans and animals under certain conditions.Conclusion A variety of bacteria may be carried by frozen intestines, the relevant supervision and study should be further strengthened.