ObjectiveA quantitative detection method for Campylobacter jejuni was developed to provide accurate data for food safety risk assessment. MethodsBased on the principle of Most Probable Number (MPN) and ISO/TS 10272-3and GB/T 4789.9-2008, fresh chickens and chicken faeces with spiked sample were cultivated in different enrichment cultures, cultivation environment and methods, the optimization was verified by standard recovery test. Results10cfu/g Campylobacter jejuni as spiked samples, 12.26and 10.96MPN/g were detected in Preston broth of fresh chickens and chicken faeces, while 2.38and 2.32MPN/g were detected in Bolton broth which had significant difference (P＜0.05). The spiked sample was detected as 12.26and 12.12MPN/g, 12.26and 10.96MPN/g, 12.26and 12.86MPN/g, and 10.82and 12.12MPN/g with three gases-incubator, gas-producing bags, gas extract-exchange, and candle-cylinder, respectively. No significant difference was found between the three-gas incubator method and the other three (P＞0.05). The spiked samples were detected as 15.8and 15.2MPN/g in static culture, 11.4and 8.7MPN/g in shaking culture, and there was significant difference between them (P＜0.05). The recoveries were 115.25%, 111.5% and 95.0% with different spiked concentrations. ConclusionThe method could be applied to detect the Campylobacter jejuni specifically, quantitatively and accurately in seriously contaminated samples. \=