The establishment of indirect ELISA method of β-lactamase in milk
DOI:
Author:
Affiliation:
Clc Number:
Fund Project:
Article
|
Figures
|
Metrics
|
Reference
|
Related
|
Cited by
|
Materials
|
Comments
Abstract:
To establish an indirect ELISA method of β-lactamase detection in milk.MethodsThe Elisa method of Ampicillin was set up and optimized with ampicillin-HRP conjugate and Ampicillin antibody. The method was used to detect the ampicillin in samples and negative control, and β-lactamase was determined indirectly by comparing OD450 values. ResultsThe indirect ELISA method for β-lactamase was established, and the sensitivity was 0.0005IU/ml.ConclusionThis method was accurate and reliable, and could be widely applied to detect β-lactamase in milk.
Reference
Related
Cited by
Get Citation
JIANG Kan, LI Yong-gang, SHEN Hong, LI Peng-peng, WU Chen-lu, JIN Yan-fei. The establishment of indirect ELISA method of β-lactamase in milk[J].中国食品卫生杂志,2013,25(5):405-409.