To establish a HPLC-MS/MS method for simultaneous determination of eight artificial sweeteners in flavorings. Methods The artificial sweeteners, including acesulfame-K, cyclamate, saccharin, aspartame, alitame, neotame, sucralose and neohesperidine dihydrochalcone were extracted with water and the water-soluble macromolecular compounds in flavorings were precipitated with methanol. The supernatant was cleaned-up using aluminum-N cartridges and separated on a C18 column using 10 mmol/L ammonium formic acid/acetonitrile as mobile phase and then detected by HPLC-MS/MS using multiple reaction monitoring (MRM) in negative ionization mode. Results The calibration curves showed a good linearity with correlation coefficients＞0.990. The linear range for saccharin was 0.05-5 μg/ml, 0.1-10 μg/ml for sucralose, and the other sweeteners was 0.01-1 μg/ml. The average recoveries were from 82.7% to 117.9%, and the relative standard deviations (RSD) were from 0.6% to 10.6%. The detection limits for liquid and semi-solid sweeteners were from 0.01 to 0.1 mg/kg and 0.1 to 1 mg/kg for solid sweeteners. Conclusion The method was specific, sensitive, easy, fast and suitable for the confirmation and quantification of 8 artificial sweeteners in flavorings.