To determine chemical toxins in food poisoning and its contents fast and accurately, and provide a scientific basis for emergency response. Methods According to the investigation and processing principles for food poisoning, HPLC with column derivatization reaction and fluorescence detection were used for 2suspected food poisoning samples sent by local CDC following NY/T 761-2008. Results High concentrations of carbofuran(27.0and 5.4g/kg)were detected in white noodles and fried egg noodles vermicelli.Conclusion This chemical poisoning case was caused by carbofuran．