A comprehensive analytical method based on UPLC-MS/MS was established for determination of sodium cyclamate in 30wine samples from markets. Methods UPLC separation was performed on an Acquity UPLCTM BEH C₁₈ column (2.1mm×100mm,1.7μm) with methanol-0.1% acetic acid(90+10) as mobile phase. Triple-quadrupole mass spectrometry analyzer was used for the qualitative and quantitative analysis. Results The limit of detection of sodium cyclamate was 10μg/L.The recoveries of Sodium cyclamate were 75.0%-105%, and the RSD was 10.5%-12.1%. Among 30wine samples, 13samples were found to contain sodium cyclamate, whose level was 0.027-891μg/ml. Conclusion The method is simple with high sensitivity and selectivity and suitable for the determination of sodium cyclamate in wine. The results indicated the presence of abuse of sodium cyclamate.