To observe the effects of strontium mineral water (SMW) on proliferation and secretion of human umbilical vein endothelial cells (HUVEC) at different strontium concentration. [WT5"HZ]Methods HUVEC were cultured in vitro. After culturing in a normal Dulbecco''s Modified Eagle''s Medium (DMEM) for 18hours, the cells were transferred to the conditioned medium with 2,4,6,8mg/L strontium mineral waters and double distilled water (DDW) treatment. The proliferation of cells was observed by Cell Counting Kit-8(CCK-8) assay at 24th, 48th, 72nd and 96th hours. Cell growth was observed by hematoxylin staining at 72nd hours. Meanwhile, cell protein was measured by Enhanced BCA Protein Assay kit and levels of extracellular vein endothelial growth factor (VEGF), nitric oxide (NO) and endothelin (ET) were tested by enzyme-linked immuno sorbent assay (Elisa). [WT5"HZ]Results The proliferation activity of cells in 2and 4mg/L SMW groups were significantly increased (P＜0.05), while significantly decreased in 8mg/L SMW group (P＜0.05) after 72hours. Total of extracellular VEGF in SMW groups were lower than DDW group (P＜0.05), and there was no significant difference between SMW groups (P＞0.05) after 72hours. NO and ET of cells in 2and 4mg/L groups were hyposecretion, while 6and 8mg/L groups were on the contrary. NO/ET ratio of 4mg/L SMW group was higher than the others (P＜0.05), and the difference of NO/ET ratio between the other groups was not significant (P＞0.05). [WT5"HZ]Conclusion A certain concentration of strontium in mineral water is good for proliferation of HUVEC. It could affect HUVEC secretion function of ET and NO, and decrease vascular tone. VEGF was not the direct factor which enhanced the proliferation activity of HUVEC culturing in strontium mineral water.