To evaluate the allergenic potential of recombinant human lactoferrin (rhLf) expressed by genetically modified cow in milk.Methods Bioinformatics searches and digestibility assay were conducted to investigate the allergenic potential of rhLf. [WT5"HZ]Results Bioinformatics searches showed that there was high-level similarity between rhLf and some known allergens, such as lactotransferrin and ovotransferrin in amino acid sequence, and Asp f 2, Ole e 10and Ole e 9in structure. Digestibility assay showed that rhLf broke down into small fragment rapidly during gastric fluid digestion, while it was resistant to intestinal fluid digestion.ConclusionRhLf has allergenic potential.