Based on GICA technology, we developed a four-in-one colloidal gold test strip for rapid detection of nitrofuran metabolites in aquatic products. MethodsGoat anti-mouse IgG, artificially conjugated antigen and the gold labeled antibody were coated onto nitrocellulose membrane and micropores, the concentration and the amount of coating were optimized, and the sample pad, the colloidal gold conjugate pad, the absorbent pad and other accessories were then assembled into test strip. ResultsThe results showed that the detection limits for aquatic AOZ, SEM, AMOZ and AHD were 1.0,1.0,1.0and 2.0μg/kg, respectively.ConclusionThe test strip is rapid,accurate and high sensitivity,suitable for the detection of nitrofuran metabolites in the large of aquatic samples.