Objective To develop a high performance liquid chromatographic-diode array detector for the simultaneous determination of Lemon Yellow,Carmine,Sunset Yellow,Allura Red and Bright Blue in fermented flour products.Methods The separation was performed on a Kromasil C18 column(250 mm×4.6 mm,10 μm) with methanol/acetonitrile(1∶ 1,V/V) and ammonium acetate(0.01 mol/L,pH=4.0)as the mobile phase at the flow rate of 1.0 ml/min.The detection wavelength was 254 nm and the peaks of UV spectra were captured by a photodiode array detector(DAD).Results The method showed a good linearity in the range of 1.56-50.0 μg/ml(r=1.000 0).The average recoveries of colorants ranged from 98.9% to 101.6%,with RSDs of 0.11%-0.41%.Conclusion The method is simple,accurate,sensitive and reliable.