Objective To investigate the antibiotic resistance and molecular types of S.wandsworth strains isolated in Shanghai from 2009—2011.Methods K-B method recommended by WHO was used to detect 6 strains of S.wandsworth.Results were analyzed according to the breakpoints of CLSI 2010;Pulsed field gel-electrophoresis(PFGE) was used for molecular typing.Results One strain of S.wandsworth was resistant to trimethoprim and tetracycline.The others were sensitive to 10 kinds of antibiotics.Six strains of S.wandsworth were classified to 5 PFGE patterns and two of them were the same pattern.Conclusion S.wandsworth strains isolated from 2009 to 2011 were highly sensitive to antibiotics,and some showed higher homology.