Comparison of methods detecting Escherichia coli 0157: H7 in foods
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R155

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    Abstract:

    To examine the specificity and sensitivity of traclitional cultural method, immunomagnetic heads separation method (IMS) , VIDAS, BAX and Loop-mediated isothermal amplific:ation ( LAMP) method for the detection of 0157: H7 in foods. Methods Traditional cultural method, IMS, VIDAS, BAX and LAMP method were used and compared in detecting 0157: H7 in pork samples. Results The detection rate of BAX, LAMP, IMS and VIDAS method was 89. 1% , 85.9% , 75. 0% and 78. 1% respectively, while that of traditional cultural method was only 43. 8%. Conclusion BAX and LAMP mObjectiveethod were proved to be rapid, highly effective, specific and sensitive for the detection of 0157: H7 in foods.

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Zhou Yong, Zhang Jing, Hou Shuiping, Deng Zhiai, Wu Xinwei, Chen Shouyi, Chen Shouyi. Comparison of methods detecting Escherichia coli 0157: H7 in foods[J].中国食品卫生杂志,2012,24(1):27-29.

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