Objective A high performance liquid chromatography(HPLC) coupled with hydride generation and atomic fluorescence spectrometry is proposed for the determination of methyl mercury in seafood.Methods Samples were extracted by 25% potassium hydroxide and the methylmercury-thiol complex was separated on a Supelco C18(150 mm×4.6 mm,5 μm) reverse phase column and online UV-digested.After reduced to element Hg by KBH4,the element Hg was selectively detected by AFS in an Ar/H2 flame under optimized condi...