Objective To establish an ultraperformance liquid chromatography with electrospray tandem mass spectrometry(UPLC-MS/MS) method for the determination of cholesterol in hotpot oil,hogwash oil and waste oil.Methods Oil samples were separated on a Waters ACQUITY UPLC BEH C18 column(2.1 mm × 50 mm,1.7 μm) with a mobile phase of methanol + formic acid(999+1).Cholesterol and internal standard(2,2,3,4,4,6-d6 cholesterol) were detected by multi-reactions monitoring(MRM) technology on the transitions of ...