Objective To develop a rapid chemiluminescence enzyme immunoassay(CLEIA) for detecting enrofloxacin(ENR) in milk.Methods Anti-ENR antibody was passively adsorbed onto the walls of polypropylene plates.ENR conjugated with labeled alkaline phosphatase(ALP) was used to compete with the ENR in milk for the limited specific capability of ENR antibody on plates by solid-phase antibody binding reaction.Through separating unbounded labeled antigen and determining of chemiluminescence,the content of the ...