Objective To develop a specific PCR detection method for L.monocytogenes and to investigate the contamination of L.monocytogenes in different foods in Chengdu city.Methods A total of 135 random samples from sorts of meat,fish,dairy,vegetable and other ready-to-eat food were collected from free markets and supermarkets.The samples were followed an enrichment period with LB1 and LB2 broth,then separated and identified by using PALCAM medium,TSA-YE agar and CHROMagarTM Listeria.The virulence gene i...