The purpose of this study is to establish a new technique for detection Vibrio perahaemolyticus (VP) faster and validate its practicability. The new technique involves designing a primer pair targeting tl gene and using the primer pair for PCR amplification The technique was tried in 14 VP strains and 30 non-VP strains, and also in factitiously contaminated food samples. The results showed that the technique offered excellent differentiation and lower detection limit (10 CFU/g). The full course of assay could be completed in 13 hours, which was significantly shorter than the time needed by conventional techniques. It is concluded that the technique is practical with advantages of simple operation, higher specificity, less time-consuming and lower detection limit. Nevertheless, more trial is needed because the technique was validated in only 2 kinds of actual food samples.