To establish the parameters for the effect of health foods on climacteric syndrome, a health food was administered by oral to ovariectomized, old non-reproductive and young mice for 30 days. Hormone and sexual organ changes were examined. The results showed that the health food at high dose enhanced significantly serum 17 beta-estradiol (E2) level, uterus weight and water uptake, and vigina expressed keratogenous changes,in its epitheium. Old non-reproductive mice were most sensitive. The results suggested that serum 17 beta-estradiol (E2) concentration, uterus weight and water uptake and vagina changes in old non-reproductive mice may be proposed as the evaluation parameters for the effect of health foods to improve climacteric syndrome.