Objective To analyze the contamination, drug resistance and genetic characteristics of Vibrio parahaemolyticus isolated from aquatic products in Jiangxi Province in 2023. Methods? A total of 212 aquatic products sold in Jiangxi Province in 2023 were collected, and the contamination of Vibrio parahaemolyticus in different seasons and different kinds was analyzed. The STs, virulence gene, resistance gene and antibiotic sensitivity of Vibrio parahaemolyticus were also detected. Results A total of 45 strains of Vibrio parahaemolyticus were detected from 212 samples with a detection rate of 21.23 %. The detection rate of Vibrio parahaemolyticus in marine products and freshwater products were 26.67 % and 15.89 %, respectively, while there was no significant difference in detection rate ( χ2 = 3.682, P > 0.05). The detection rate in the third quarter was highest (22.62 %). The detection rate in crustacea and shellfish were highest，both of which were 25.00 %. A total of 40 STs were found in 45 isolates, of which 17 were newly discovered STs, and no dominant STs were found.The phylogenetic analysis of cgMLST showed that the 45 isolates were distantly related. All 45 isolates carried tlh and T3SS1 genes, only 2 isolates carried trh gene, and tdh and T3SS2 genes were not detected.The 45 isolates presented highly resistant to cefazolin ( 100.00 % ) and polymyxin E ( 51.11 % ), and the multiple drug-resistant strains were found. A total of 13 drug resistance genes were detected, including CARB-20, tet(35), sul1, sul2, dfrA6, CRP, QnrS2, ANT(2")-Ia, ACC(6")-Ia and tet(E). The carrying of resistance genes is not completely consistent with the resistance phenotype. Conclusion The aquatic products sold in Jiangxi province were polluted by Vibrio parahaemolyticus at a certain degree. TheVibrio parahaemolyticus strains has rich genetic diversity and presented a distantly related. The carrying rate of virulence gene was low. The multiple drug-resistant strains were found, and the drug resistance mechanism needed to further studied because of its complexity.