Objective To understand the mold and mycotoxin contamination status of spices sold in Guizhou province, and to provide scientific basis for formulating pollution control measures. Mthods A total of 260 samples of sold spice were collected from 2022 to 2023 in Guizhou province. Mold counting of these samples were detected according to the national food safety standard (GB 4789.15), and isolated molds were identified using morphology and mass spectrometry methods. Fifty-two mold positive samples were randomly selected to detecte aflatoxin B1, B2, G1, G2 and ochratoxin. Results 72.31% (188/260) of the samples were contaminated with mold, with an average contamination level of 5.1×103 CFU/g. Molds were detected in all types and different packaging types of spice samples. There was a statistically significant difference in the detection rate of samples collected in different quarters (P<0.05). The samples mainly contaminated mold of genera Aspergillus, Rhizopus, Mucor and Penicillium, of which 34.14% (155/454) were toxin producing mold. The total detection rate of mycotoxins was 48.08% (25/52), mainly including AFTB2 and OTA. Two or more mycotoxins were detected simultaneously in 13.46% (7/52) of the samples. The detection rate of AFTG1 in prepackaged samples was significantly higher than that in bulk samples. The exceeding rate of AFTB1 was 5.77% (3/52). Conclusion There was mold and mycotoxin contamination in spices sold in Guizhou Province. It is recommended to strengthen the monitoring and management of spices to provide scientific basis for risk assessment and developing relevant standards.