Objective:Through the rapid detection and analysis of a case of gelsemine?? poisoning caused by honey, and the incident disposal process was reviewed to bring inspiration for similar incident handling. Methods: The appropriate amount of samples were weighed or aspirated, adding borax-NaOH buffer solution(pH=9.6), shake well, extracted with ethyl acetate, and the organic layer was used for gas chromatography-mass spectrometry detection and analysis. Results: The gelsemine content in the honeycomb was 28.6mg/kg, and the gelsemine contents in tool 1 and tool 2 were 5.85μg and 5.04μg. The gelsemine content in honey water was 44.0mg/L, and only the blood samples of patients 1-3 detected gelsemine content of 22.3μg/L-402μg/L. The urine of all patients were detected gelsemine content of 20.6μg/L-299μg/L, and the urine gelsemine content of patient 2-6 was higher than that of blood. Conclusion: This food poisoning was caused by honey containing gelsemine, with low poisoning dosage and which can be detected from tools.The kidney is one of the metabolic pathways of gelsemine. After 2 hours and 45 minutes of consumption, most patients had higher urine gelsemine content than blood, indicating that urine sample collection should be emphasized.