Objective To establish a digital polymerase chain reaction(PCR)method for hepatitis A virus (HAV) in shellfish and berry foods. Methods After sample enrichment by proteinase K digestion polyethylene glycol method, RNA was extracted by high purity virus nucleic acid kit, and then digital PCR was used to detect HAV. Results This method had typical amplification, good repeatability and stability for HAV. The sensitivity of HAV in strawberry, raspberry and shellfish samples was 25.30 CCID₅₀/20 g, 6.32 CCID₅₀/20 g and 12.54 CCID₅₀/2 g respectively, which means that the detection sensitivity of HAV was high. Conclusion This method is rapid, accurate, sensitive, and is suitable for the determination of HAV in shellfish and berry foods.